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Abstract Light emission from the North American firefly Photinus pyralis, which emits yellow-green nm light, is widely believed to be the most efficient bioluminescence system known, making this luciferase an excellent tool for monitoring gene expression. National Center for Biotechnology Information , U. Gov't Research Support, U. National Center for Biotechnology Information , U. Starting with the luciferase variant SerThr, we introduced the mutations ThrAla, AlaLeu, IleAla, PheLeu, and GluLys to produce a new red-emitting enzyme with a bioluminescence maximum of nm, narrow emission bandwidth, favorable kinetic properties, and excellent thermostability at 37 degrees C. Generate a file for use with external citation management software.
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Targeting Systems || Products - Drug Discovery - Luciferase Assay Reagents


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In vivo bioluminescence for tracking cell fate and function

For example, by linking the expression of luciferase to the cardiac-specific promoter myosin light chain 2v, it is possible to monitor cells undergoing cardiac differentiation over time via the detection of reporter gene expression by BLI 27 , A method for assaying the presence of ATP in a sample, the method comprising the steps of: The bathochromic shift observed in alkaline conditions results from formation of the oxyluciferin dianion. Continue with Google.
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